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KMID : 0380620150470040525
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Korean Journal of Food Science and Technology
2015 Volume.47 No. 4 p.525 ~ p.533
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Anti-adipogenic Effects of the Water Extracts of Defatted Green Tea Seed Cake
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Wee Ji-Hyang
Sung Hea-Mi
Jung Kyung-Ok
Kim Suk-Jung
Shin Yu-Rim
Park Ju-Hyun
Kim Jong-Deog
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Abstract
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mechanisms of lipolysis in mature 3T3-L1 adipocytes were investigated. In this study, we found that the naringenin content of GTSE was 5.5 mg/g; however, catechins were not detected. The intracellular lipid droplets were stained with Oil Red O dye and quantified. Compared to the control, lipid accumulation was significantly decreased by 52%, and intracellular triglyceride (TG) level was reduced by 33% after treatment with GTSE at a concentration of 40 μg/mL. To determine the mechanism of reduction in TG content, we determined the level of fatty acid synthase (FAS), phosphorylation of adenosine monophosphate-activated protein kinase (AMPK), and acetyl-coenzyme A carboxylase (ACC) in the cell model. Incubation of the 3T3-L1 adipocytes with GTSE stimulated AMPK and ACC phosphorylation in a dosedependent manner, and decreased the expression of FAS.
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KEYWORD
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Defatted green tea seed cake, naringenin, 3T3-L1 adipocyte, lipolysis
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